1 |
General information |
a |
Purpose of study |
b |
Description of endpoints |
2 |
Test setup |
a |
Performed according to standard/modified standard (e.g., OECD, US EPA) |
b |
Performed according to Good Laboratory Practices (GLP) |
c |
Description of used control(s): dispersion control, metal ion control, bulk particle of the same chemical composition, negative and positive control |
d |
Control(s) mortality, growth, morbidity, physical effects, shading and other observed non-standard effects like behavior and coloring |
e |
Comparison to validity criteria (e.g. control survival, growth) from appropriate standard test method |
3 |
Test compound |
a |
Identification (e.g. name, CAS-number, particle size, shape, particle size distribution, surface area, coating) |
b |
Physico-chemical characteristics that may influence the behavior of the nanomaterial during the study data (e.g. solubility, aggregation, agglomeration) |
c |
Source (supplier, batch number) |
d |
Purity in % |
e |
Composition of product formulation, and presence of impurities |
4 |
Test organism |
a |
Scientific name |
b |
Body weight, length |
c |
Age/life-stage |
d |
Growth/reproductive condition |
e |
Sex |
f |
Strain, clone |
g |
Source, including possible pre-exposure for field-collected species |
h |
Culture handling |
5 |
Exposure conditions |
a |
Exposure schedule (static, semi static, flow through system, other) and flow-rate (flow-through systems) or renewal time (semi-static systems) |
b |
Open or closed system |
c |
Test medium composition and source of test water (e.g., well water, deionised water, tap water) |
d |
Temperature, and time-points for measuring |
e |
pH, and time-points for measuring |
f |
Hardness of water, and time-points for measuring |
g |
Conductivity, and time-points for measuring |
h |
Dissolved oxygen content, and time-points for measuring |
i |
Light intensity and quality (source and homogeneity), light/dark conditions |
j |
Feeding protocols, food composition |
k |
Material and volume of aquarium/container and other equipment in contact with test organisms and nanomaterial |
l |
Use of sand or sediment, and its characteristics (total organic carbon (TOC), particle size, etc.) |
m |
Preparation of stock suspension, including solvent concentrations in test water and controls |
n |
Nominal concentrations of nanomaterial |
o |
Measured concentrations and physical, chemical, or biological transformations of the tested nanomaterial, and time-points for measuring |
p |
Analytical method: description of method, including limit of detection (LOD), limit of quantification (LOQ) |
q |
Exposure duration and total test duration |
r |
Time-points of observations for endpoints |
s |
Results based on nominal or measured concentrations taking potential instability of suspensions into account |
t |
Biomass loading (nanomaterial to biomass ratio) |
6 |
Statistical Design and Biological Response |
a |
Number of replicates for control(s) and test concentrations; setup of replicates (avoid pseudo-replication) |
b |
Number of organisms, or algal cell concentration, per replicate |
c |
Treatment design (e.g., block, randomized) |
d |
Statistical method used |
e |
Biological response for each concentration |
f |
Dose-response observed |
g |
Statistically significant responses noted (e.g. ECx) |
h |
Significance level for NOEC and LOEC data (0.05 or less) |
i |
Estimation of variability for LCx and ECx data |
j |
Availability of raw data: through supplementary information, a website, or upon request.
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